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4-Aminopyridine (4-AP) calculator

Prepare a stock, plan an experiment, and allow for pipetting losses.

Drug

Nonselective voltage-dependent K⁺-channel blocker

Formula: C₅H₆N₂
Molecular weight: 94.12 g/mol
Solvent: DMSO

Stock and experiment

Working concentration (µM)Stock concentration factor (×)Final aCSF recording solution volume (mL)Number of aliquots

Aliquot handling allowance (%)

About this allowance

This extra volume allows for pipetting losses.

Stock concentration = working concentration × factor ÷ 1000 (mM). Each aliquot supplies one experiment plus the handling allowance.

Calculated results

Stock concentration: 500 mM
Total powder required: 25.883 mg
Total stock volume: 550 µL
Volume per aliquot including handling allowance: 55 µL
Stock volume to add per experiment: 50 µL

The allowance increases aliquot volume, not concentration. Use the volume to add for the experiment; the excess is for pipetting losses.

EphysCalc · Drug preparation

4-Aminopyridine (4-AP)

Nonselective voltage-dependent K⁺-channel blocker

Formula: C₅H₆N₂ · Molecular weight: 94.12 g/mol

Solvent: DMSO

Parameter	Value
Working concentration	500 µM
Stock concentration factor	1,000×
Stock concentration	500 mM
Final aCSF recording solution volume per experiment	50 mL
Number of aliquots	10
Aliquot handling allowance	10%
Total powder required	25.883 mg
Total stock volume	550 µL
Volume per aliquot including allowance	55 µL
Stock volume to add per experiment	50 µL

The handling allowance is included in the total powder and stock volume. Add only the listed stock volume per experiment; the aliquot excess allows for pipetting losses.

ephyscalc.com · Drug stock calculator