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Ephys solution calculator
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Sucrose Cutting Solution
Low-Na⁺, high-Mg²⁺/low-Ca²⁺ formulation designed to protect tissue during sectioning. Evidence & sources →
Powder · weigh Stock · pipette
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Sucrose cutting solution for brain slices
Sucrose-based cutting solutions are used during brain-slice preparation and have a different purpose from recording aCSF. Follow the slicing and recovery conditions established for your preparation, including temperature, oxygenation, and the transition to holding or recording solution.
Aghajanian and Rasmussen (1989): adult brain-slice preparationExternal solution recipe
Sucrose Cutting Solution
External solution · slicing
Final volume1000.0 mL
| Compound | Final (mM) | MW (g/mol) | Source | Stock (mM) | Add |
|---|---|---|---|---|---|
| Sucrose | 190 | 342.30 | Powder | — | 65.04 g |
| KCl | 1.9 | 74.56 | Stock solution | 1,000 | 1.90 mL |
| NaH₂PO₄ | 1.2 | 137.99 | Stock solution | 1,000 | 1.20 mL |
| NaHCO₃ | 25 | 84.01 | Powder | — | 2.10 g |
| CaCl₂ | 0.3 | 111.00 | Stock solution | 1,000 | 0.30 mL |
| MgCl₂ | 6 | 203.30 | Stock solution | 1,000 | 6.00 mL |
| Dextrose | 10 | 180.16 | Powder | — | 1.80 g |
| Myo-inositol | 3 | 180.00 | Powder | — | 0.54 g |
| L-ascorbic acid-Na | 2 | 198.11 | Powder | — | 0.40 g |
| Na-pyruvate | 2 | 110.00 | Powder | — | 0.22 g |
Solids to weigh70.10 g
Stocks to add9.40 mL
Nominal solute total241.4 mM