Whole-cell tools
Ephys solution calculator
LIVE CALCULATION
Current clamp
K-gluconate internal
Enter the final concentration, molecular weight, and whether each component is weighed or delivered from stock.
Powder · weigh Stock · pipette
| Compound | Final mM | MW g/mol | Source | Stock mM | Add | Remove |
|---|---|---|---|---|---|---|
Internal solution recipe
K-gluconate internal
Current clamp
Final volume50.0 mL
| Compound | Source | Final (mM) | MW (g/mol) | Add |
|---|---|---|---|---|
| K-gluconate | Powder | 128.0 | 234.25 | 1499.2 mg |
| KCl | Powder | 10.0 | 74.56 | 37.3 mg |
| HEPES | 100.0 mM stock | 10.0 | 238.30 | 5000 µL |
| EGTA | 100.0 mM stock | 1.0 | 380.40 | 500 µL |
| MgCl₂ | 1000.0 mM stock | 1.0 | 203.30 | 50 µL |
| CaCl₂ | 1000.0 mM stock | 0.3 | 111.00 | 15 µL |
| Na₂ATP | Powder | 5.0 | 507.18 | 126.8 mg |
| NaGTP | Powder | 0.3 | 523.20 | 7.8 mg |
Solids to weigh1671.1 mg
Stocks to add5565 µL
Nominal solute total155.6 mM
Verify supplier-specific salt form, hydration state, assay, pH, final volume, and measured osmolality before use.