Whole-cell tools
Ephys solution calculator
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Voltage clamp · EPSC
EPSC Internal (low chloride)
Enter the final concentration, molecular weight, and whether each component is weighed or delivered from stock.
Powder · weigh Stock · pipette
| Compound | Final mM | MW g/mol | Source | Stock mM | Add | Remove |
|---|---|---|---|---|---|---|
Internal solution recipe
EPSC Internal (low chloride)
Voltage clamp · EPSC
Final volume50.0 mL
| Compound | Source | Final (mM) | MW (g/mol) | Add |
|---|---|---|---|---|
| Cs-methanesulfonate | Powder | 135.0 | 228.00 | 1539.0 mg |
| HEPES | 100.0 mM stock | 10.0 | 238.30 | 5000 µL |
| EGTA | 100.0 mM stock | 1.0 | 380.35 | 500 µL |
| MgCl₂ | 1000.0 mM stock | 4.0 | 203.30 | 200 µL |
| Mg-ATP | Powder | 4.0 | 507.18 | 101.4 mg |
| Na-GTP | Powder | 0.3 | 567.10 | 8.5 mg |
| Na₂-phosphocreatine | Powder | 7.3 | 255.10 | 93.1 mg |
Solids to weigh1742.1 mg
Stocks to add5700 µL
Nominal solute total161.6 mM
Verify supplier-specific salt form, hydration state, assay, pH, final volume, and measured osmolality before use.