External solution · Sucrose cutting
Sucrose cutting solution recipe
A 2 L sucrose-based cutting solution starting recipe for electrophysiology tissue preparation. Use the interactive calculator to scale the batch or edit concentrations, molecular weights, and stock solutions.
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2 L starting recipe
| Compound | Final (mM) | MW (g/mol) | Source | Add |
|---|---|---|---|---|
| Sucrose | 190.0 | 342.30 | Powder | 130.07 g |
| KCl | 1.9 | 74.56 | 1000.0 mM stock | 3.80 mL |
| NaH₂PO₄ | 1.2 | 137.99 | 1000.0 mM stock | 2.40 mL |
| NaHCO₃ | 25.0 | 84.01 | Powder | 4.20 g |
| CaCl₂ | 0.25 | 111.00 | 1000.0 mM stock | 0.50 mL |
| MgCl₂·6H₂O | 6.0 | 203.30 | 1000.0 mM stock | 12.00 mL |
| Dextrose | 10.0 | 180.16 | Powder | 3.60 g |
| Myo-inositol | 3.0 | 180.00 | Powder | 1.08 g |
| L-ascorbic acid-Na | 2.0 | 198.11 | Powder | 0.79 g |
| Na-pyruvate | 2.0 | 110.00 | Powder | 0.44 g |
About this sucrose cutting solution
This external formulation uses sucrose as its major osmotic component and includes potassium, phosphate, bicarbonate, calcium, magnesium, dextrose, myo-inositol, ascorbate, and pyruvate. It is an editable starting recipe, since cutting solutions vary between preparations and laboratory protocols.
Confirm the intended gas equilibration, pH, temperature, and measured osmolality for your preparation. Verify every salt form and hydration state against its supplier label before preparing the solution.
How the amounts are calculated
Powder
g = mM × L × MW ÷ 1000
Stock solution
mL = desired mM × final mL ÷ stock mM
Before preparing the solution
Treat this as a starting formulation. Verify the exact salt form, hydration state, molecular weight, and assay on each supplier label. Adjust pH experimentally, bring the mixture to final volume, and measure osmolality. The nominal concentration sum is not an osmolality prediction.